Publications

Research Articles

• Deciphering the TET3 interactome in primary thymic developing T cells

Dimitris Theofilatos , Tricia Ho , Greg Waitt , Tarmo Äijö, Lucio M Schiapparelli , Erik J Soderblom , Ageliki Tsagaratou. iScience,

• Protocol to isolate mature thymic T cell subsets using fluorescence-activated cell sorting for assessing gene expression by RNA-seq and transcription factor binding across the genome by CUT&RUN.

Dimitris Theofilatos , Tarmo Äijö , Ageliki Tsagaratou. STAR Protocols, 2022 Nov 11;3(4):101839. eCollection 2022 Dec 16.

 

• TET proteins regulate T cell and iNKT cell lineage specification in a TET2 catalytic dependent manner

Tarmo Äijö , Dimitris Theofilatos  , Meng Cheng, Matthew D. Smith , Yue Xiong , Albert S. Baldwin and Ageliki Tsagaratou, Frontiers in Immunology, 2022 Aug 5

 

• Paradoxical association of TET loss of function with genome-wide DNA hypomethylation.

López-Moyado IF, Tsagaratou A, Yuita H, Seo H, Delatte B, Heinz S, Benner C, Rao A. Proc Natl Acad Sci U S A. 2019 Aug 20;116(34):16933-16942.

 

• TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells.
  Tsagaratou A, Gonzalez-Avalos E, Rautio S, Scott Browne J, Togher S, Pastor WA, Rothenberg E.V., Chavez L, Lahdesmaki H, Rao A. Nature Immunology 2017 Jan; 18 (1); 45-53 

 

• A probabilistic generative model for quantification of DNA modifications enables analysis of demethylation pathways.
  Aijo, T., Huang, Y., Mannerstrom, H., Chavez, L., Tsagaratou, A., Rao, A., and Lahdesmaki, H. Genome biology 2016 Mar 14; 17:49

 

• Control of Foxp3 stability through modulation of TET activity
  Yue, X., Trifari, S., Aijo, T., Tsagaratou, A., Pastor, W.A., Zepeda-Martinez, J.A., Lio, C.J., Li, X., Huang, Y., Vijayanand, P., Lähdesmäki H, Rao A. The Journal of experimental medicine. 2016 Mar 7; 213 (3):377-97

 

• Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.
  Kang, J., Lienhard, M., Pastor, W.A., Chawla, A., Novotny, M., Tsagaratou, A., Lasken, R.S., Thompson, E.C., Surani, M.A., Koralov, S.B., Kalantry S, Chavez L, Rao A. Proceedings of the National Academy of Sciences of the United States of America 112 , (31): E4236-45

 

• Jarid2 is induced by TCR signaling and controls iNKT cell maturation.
  Pereira, R.M., Martinez, G.J., Engel, I., Cruz-Guilloty, F., Barboza, B.A., Tsagaratou, A., Lio, C.W., Berg, L.J., Lee, Y., Kronenberg, M., Bandukwala HS, Rao A. Nature communications 2014 Aug 8;5:4540

 

• Dissecting the dynamic changes of 5- hydroxymethylcytosine in T-cell development and differentiation.
  Tsagaratou, A.*, Aijo, T.*, Lio, C.W., Yue, X., Huang, Y., Jacobsen, S.E., Lahdesmaki, H., and Rao, A. Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3306-15. (*equal contribution)

 

• Inactivation of the deubiquitinase CYLD in hepatocytes causes apoptosis, inflammation, fibrosis, and cancer.
  Nikolaou, K., Tsagaratou, A., Eftychi, C., Kollias, G., Mosialos, G., and Talianidis, I. (2012). Cancer cell  2012 Jun 12;21(6):738-50

 

• Ten-Eleven- Translocation 2 (TET2) negatively regulates homeostasis and differentiation of hematopoietic stem cells in mice.
  Ko, M., Bandukwala, H.S., An, J., Lamperti, E.D., Thompson, E.C., Hastie, R., Tsangaratou, A., Rajewsky, K., Koralov, S.B., and Rao, A.. Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 30;108(35):14566-71

 

• Differential requirement of IKK2 for CYLD-dependent representation of thymic and peripheral T-cell populations.
  Tsagaratou, A., Grammenoudi, S., and Mosialos, G. European journal of immunology 2011 Oct;41(10):3054-62.

 

• Truncation of the deubiquitinating domain of CYLD in myelomonocytic cells attenuates inflammatory responses.
  Tsagaratou, A., Kontoyiannis, D.L., and Mosialos, G. 2011 Jan 20;6(1):e16397

 

• Thymocyte-specific truncation of the deubiquitinating domain of CYLD impairs positive selection in a NF-kappaB essential modulator-dependent manner.
  Tsagaratou, A., Trompouki, E., Grammenoudi, S., Kontoyiannis, D.L., and Mosialos, G. Journal of immunology 2010 Aug 15;185(4):2032-43

 

• Truncation of the catalytic domain of the cylindromatosis tumor suppressor impairs lung maturation.
  Trompouki, E., Tsagaratou, A., Kosmidis, S.K., Dolle, P., Qian, J., Kontoyiannis, D.L., Cardoso, W.V., and Mosialos, G. Neoplasia 2009 May;11(5):469-76

Review Articles

• TET proteins in the spotlight: Emerging Concepts of Epigenetic Regulation in T cell biology

Tsagaratou A. Immunohorizons (2023) 7 (1): 106–115.

 

• TET-Mediated Epigenetic Regulation in Immune Cell Development and Disease

Tsiouplis N.J., Bailey D.W., Chiou L.F., Wissink F.J., Tsagaratou A. Front. Cell Dev. Biol., 15 January 2021 

 

• Deciphering the multifaceted roles of TET proteins in T-cell lineage specification and malignant transformation

Tsagaratou, A. Immunological Reviews , 2021 Jan 7

 

• Unveiling the regulation of NKT17 cell differentiation and function.
  Tsagaratou A. Molecular Immunology, 2018 Nov 26;105:55-61

 

• TET mediated epigenetic regulation of iNKT cell lineage fate choice and function.
  Tsagaratou A. Molecular Immunology  2018 Aug 31;101:564-573.

 

• TET Methylcytosine Oxidases in T Cell and B cell Development and Function.
  Tsagaratou A. Lio CJ, Yue X, Rao A. Frontiers in Immunology. 2017 Mar 31;8:220

 

• TET Proteins and 5-Methylcytosine Oxidation in the Immune System.
  Tsagaratou A & Rao A Cold Spring Harbor symposia on quantitative biology. 2013;78:1-10.